locus

BSU00120

BSU_00120

outlinks

bsu

BSU00120

BSU_00120

The protein

Protein family

glutamine amidotransferase PdxT/SNO family (according to UniProt)

Glutaminase PdxT/SNO family (single member, according to UniProt)

Biological materials

Mutant

BKE00120 ([[gene|pdxT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT, downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG

BKK00120 ([[gene|pdxT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT, downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG

BV604 (([[gene|pdxS]]-[[gene|pdxT]])::tet), available in [SW|Fabian Commichau]'s lab, [Pubmed|24972371]

BKE00120 ([[gene|pdxT]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT, downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG

BKK00120 ([[gene|pdxT]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00120 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGTCAGCAGCGCTCCTAT, downstream forward: _UP4_TAAAACAGTTGAAAGCTGTG

BV604 (([[gene|pdxS]]-[[gene|pdxT]])::tet), available in [SW|Fabian Commichau]'s lab, [Pubmed|24972371]

BP1100 (([[gene|pdxS]]-[[gene|pdxT]])::tet) trp⁺), available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

BP1105 (([[gene|pdxT]])::cat) trp⁺), available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

Biological materials

Expression vector

pBP767: IPTG inducible expression, purification in E. coli with cleaveable N-terminal His-tag, in petSUMOadapt, available in [SW|Fabian Commichau]'s and [SW|Jörg Stülke]'s labs

pBP772: expression of ''pdxST''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

pBP774: IPTG inducible expression and purification of ''pdxST'' in ''E. coli'' with C-terminal Strep-tag, in [SW|pGP574], available in [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs